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Immunohistochemical observation of local inflammatory cell infiltration in the host-tissue reaction site of human hydatid cysts

Published online by Cambridge University Press:  30 April 2018

R. Jafari ,
B. Sanei ,
A. Baradaran ,
M. Kolahdouzan ,
B. Bagherpour  and
H. Yousofi Darani
R. Jafari
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
B. Sanei
Affiliation:
Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
A. Baradaran
Affiliation:
Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
M. Kolahdouzan
Affiliation:
Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
B. Bagherpour
Affiliation:
Acquired Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
H. Yousofi Darani*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
*
Author for correspondence: H. Yousofi Darani, E-mail: yousofidarani@gmail.com
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Abstract

The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis. In the host-tissue reaction site of liver hydatid cysts, a distinct pattern of local immune cell response, which outwardly consisted of a pack of the fibrous elements, a layer of palisading macrophages, an eosinophil-containing layer and a layer of accumulated lymphocytes, was observed. However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells. The CD3+ T cells were the predominant inflammatory cells in all groups, followed by CD19+ B cells. It can be concluded that different immune cells are involved in the local response to human hydatid cysts.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 3 , May 2019 , pp. 277 - 285
Copyright
Copyright © Cambridge University Press 2018 

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Footnotes

*

Current address: Infectious Disease and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

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